1-Coating buffer (PH 9.6)
1.59 g carbonate de sodium (Na2CO3)
2,93 g bicarbonate de sodium (NaHCO3)
0,20 g sodium azide (NaN3)
Dissoudre dans 900 ml d’eau, ajuster le pH à 9,6 avec HCl jusqu’à 1 L
2-PBS (PH 7.4) phosphate buffer saline
8.0 g Sodium chloride (NaCl)
0.2 g monobasic potassium phosphate (KH2PO4)
1, 15 g dibasic sodium phosphate Na2HPO4)
0.2 g potassium chloride (KCl)
Dissolve in 900ml H2O, ajust pH to 7,4 with NaOH or HCl and make up to1L.
3- PBS-Tween (PBST)
PBS + 0.5ml Tween 20 per liter
4-Sample extraction buffer (pH 7.4)
PBST + 2% PVP (Sigma PVP-40 polyvinyl pyrrolidone) +0.02M Sodium Sulfite (Na2SO3)
5-Conjugate buffer
PBST + 2% PVP + 0.2% egg albumin (Sigma A-5253)
6-Substrate buffer
97 ml diethanolamine
600 ml H2O
0.2 g sodium azide (NaN3)
Ajust to pH 9.8 with HCl and make up to 1 liter with H2O.